| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157459 |
| Chromosome: | chromosome_9 |
| Location: | 3271061 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g387986 | antisense | 3'UTR|outside_mRNA | ||
| Cre09.g388023 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTCCATCACTCTAGACACCAATACACCCCT |
| Internal bar code: | GTTCAGACATTGGCCGCGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 484 |
| LEAP-Seq percent confirming: | 98.9637 |
| LEAP-Seq n confirming: | 2292 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACACTGGCAGAACTGGA |
| Suggested primer 2: | CCCAAAAACAACAACTTGGG |