| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.157459 |
| Chromosome: | chromosome 9 |
| Location: | 6740093 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409050 | OTU6 | (1 of 2) K13719 - ubiquitin thioesterase OTU1 (OTU1, YOD1); OTU-like cysteine protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAGGGAGGGAGGAGGGGGGGGAGGAGGT |
| Internal bar code: | AAAACAGGTCATGCGGGCATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 688 |
| LEAP-Seq percent confirming: | 99.8985 |
| LEAP-Seq n confirming: | 984 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAACAGCTGCCTGTTCAA |
| Suggested primer 2: | ACCCACTCACCCTTGTCTTG |