| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157469 |
| Chromosome: | chromosome 7 |
| Location: | 3904692 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g339104 | (1 of 2) 3.6.3.19 - Maltose-transporting ATPase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGGCGGGCAGCGCTGCACAGCCGGCTT |
| Internal bar code: | ATCCACGACTTGGTCGGTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1151 |
| LEAP-Seq percent confirming: | 99.4964 |
| LEAP-Seq n confirming: | 14619 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGTGCGGTATTTGCCTT |
| Suggested primer 2: | GCTGCTGAAACAACACTGGA |