| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157469 |
| Chromosome: | chromosome 12 |
| Location: | 3518866 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513150 | MIA40 | (1 of 1) K17046 - protein DEK (DEK) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGGTAGGCAGGTCAGATGGGTGTCCACG |
| Internal bar code: | GTGGCGCCCCCTTGTTTAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 763 |
| LEAP-Seq percent confirming: | 99.669 |
| LEAP-Seq n confirming: | 3011 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACGTTCGAACTAGCCAGC |
| Suggested primer 2: | CAAGTAGGAGTCCTGCAGCC |