| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157500 |
| Chromosome: | chromosome 17 |
| Location: | 6834646 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g745197 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCCGCAGCAAGGCACGGCACTGCACGC |
| Internal bar code: | TGGTTTGGTGACGCCGTGACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 864 |
| LEAP-Seq percent confirming: | 89.1467 |
| LEAP-Seq n confirming: | 6571 |
| LEAP-Seq n nonconfirming: | 800 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGAGCCTACCAGAAGACG |
| Suggested primer 2: | ATCCAAGCTGGATCTGCATC |