Insertion junction: LMJ.RY0402.157504_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.157504
Chromosome:chromosome 9
Location:7089452
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g411633 (1 of 4) PF02135 - TAZ zinc finger (zf-TAZ) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGCGGTCACAGGTCGTCGCAGTCAGGCC
Internal bar code:GGGGGATCGCGCGGAACAGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:873
LEAP-Seq percent confirming:99.7427
LEAP-Seq n confirming:2326
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:TGATCCTGTTGCTGCTGTTC
Suggested primer 2:CCACCCGTTTGTCTCTGTTT