| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.157510 |
| Chromosome: | chromosome_2 |
| Location: | 5149228 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g105300 | ANK19,FAP231 | Flagellar Associated Protein with ankyrin repeats | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGACGCTCACAAGAACCGCGAGCCGATGCG |
| Internal bar code: | GTAGCACGCGCTCGGACGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 638 |
| LEAP-Seq percent confirming: | 99.9045 |
| LEAP-Seq n confirming: | 3137 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCCCAAACTAAACCAAA |
| Suggested primer 2: | CTTGTGAGCAGAGGGGCTAC |