| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.157512 |
| Chromosome: | chromosome 1 |
| Location: | 3240837 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g020264 | VPS30,ATG6,APG6 | (1 of 1) K08334 - beclin 1 (BECN1, VPS30, ATG6); Autophagy-related protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAACCAAGCGGAACTAGTTGAGCACAGGC |
| Internal bar code: | GGGCAGGGACGTGGTCGCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 177 |
| LEAP-Seq percent confirming: | 99.4267 |
| LEAP-Seq n confirming: | 2081 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTACCCCTGCTTGGTCCT |
| Suggested primer 2: | TTGCAAATGAGCTTGTACGG |