| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157539 |
| Chromosome: | chromosome 9 |
| Location: | 2812597 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388850 | FAP329,ACA1 | Flagellar Asociated Protein 329; (1 of 1) PF00122//PF00689//PF00690//PF12710 - E1-E2 ATPase (E1-E2_ATPase) // Cation transporting ATPase, C-terminus (Cation_ATPase_C) // Cation transporter/ATPase, N-terminus (Cation_ATPase_N) // haloacid dehalogenase-like hydrolase (HAD) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGGAGAAGCCCCCATACCGGCTCGGCGT |
| Internal bar code: | CTGCCGAGCGAGTAGCGTGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 926 |
| LEAP-Seq percent confirming: | 91.1589 |
| LEAP-Seq n confirming: | 3351 |
| LEAP-Seq n nonconfirming: | 325 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTGCATTCATCCGTTGT |
| Suggested primer 2: | CATGTGACACGACACTTCCC |