| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.157555 |
| Chromosome: | chromosome 8 |
| Location: | 4858351 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g384700 | DMA3 | (1 of 1) PF06325 - Ribosomal protein L11 methyltransferase (PrmA) (PrmA); Putative DNA methylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCGCTCCTGCCCCCCCCTGCGCCCTGC |
| Internal bar code: | CTTTTAACTCCTGCATTTTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 424 |
| LEAP-Seq percent confirming: | 75.2941 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTGGAGCTCTTGTGTA |
| Suggested primer 2: | GCAATTGTTTATGTGCACGG |