Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.157556 |
Chromosome: | chromosome 10 |
Location: | 4182280 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g449850 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCTGCGCCGCCGCTGCCAAGCCCTGTC |
Internal bar code: | GACTAGCCTCGTATCGCGCGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 374 |
LEAP-Seq percent confirming: | 96.6589 |
LEAP-Seq n confirming: | 1244 |
LEAP-Seq n nonconfirming: | 43 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATCAAAACACATGGCGATCA |
Suggested primer 2: | AGCTACTGCCGAATGATGCT |