Insertion junction: LMJ.RY0402.157598_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.157598
Chromosome:chromosome 15
Location:34882
Confidence (%):58
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCGGTAGAGTTCGGGAAGGGAGATGAGT
Internal bar code:GAGTAAAGACACGTGGAGGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:997
LEAP-Seq percent confirming:99.5567
LEAP-Seq n confirming:1572
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGTATCTAGCAGGGCTAC
Suggested primer 2:ACAGTCCACAGTCCCACTCC