Insertion junction: LMJ.RY0402.157613_4

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.157613
Chromosome:chromosome 9
Location:908287
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATACATTCGCCTTCCATGGGTTTCGAACC
Internal bar code:CTAGGGGGGAATTCCACACCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:92
LEAP-Seq percent confirming:71.2963
LEAP-Seq n confirming:77
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTTGGCTAGGACTCACTG
Suggested primer 2:CGTCAGGCACACAGAGACAT