| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.157701 |
| Chromosome: | chromosome_4 |
| Location: | 2574469 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g222500 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGGTATGCGCGCTGCCCTCGAGCGCGCTG |
| Internal bar code: | GTGCATCTGCCTTAGTGGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 527 |
| LEAP-Seq percent confirming: | 99.5098 |
| LEAP-Seq n confirming: | 203 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGATAGCGTTGTGCCTCTG |
| Suggested primer 2: | CCCAAGCCTATGGAGGTGTA |