| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.157709 |
| Chromosome: | chromosome 3 |
| Location: | 1965963 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155700 | (1 of 2) PTHR22840//PTHR22840:SF12 - FAMILY NOT NAMED // WD REPEAT-CONTAINING PROTEIN 36 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATACCAAAGGTGGAGGACGGGAGGAAG |
| Internal bar code: | GGCCCCTGTGCTGCATCCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1071 |
| LEAP-Seq percent confirming: | 98.6549 |
| LEAP-Seq n confirming: | 3007 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATCCAAGTTGGCAGTGTT |
| Suggested primer 2: | CACCAACAACCTCCAGACCT |