| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157734 |
| Chromosome: | chromosome 10 |
| Location: | 5263153 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457331 | (1 of 12) 2.7.13.3 - Histidine kinase / Protein kinase (histidine) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGGTAGCGGCTGACCTGTGCGGCGACA |
| Internal bar code: | TATTAGTGGTCGCAGCGGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1022 |
| LEAP-Seq percent confirming: | 99.8476 |
| LEAP-Seq n confirming: | 5240 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCACTACCTTGGGCAAC |
| Suggested primer 2: | AATTCATCCGAGACGAGGTG |