Insertion junction: LMJ.RY0402.157736_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.157736
Chromosome:chromosome 6
Location:5812303
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g287650 (1 of 1) 2.1.1.43//6.3.2.19 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase // Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCGGTGGGACACAGATGAGTGACGCGCG
Internal bar code:GCCGGGGCGCACATGGGTTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:816
LEAP-Seq percent confirming:96.8745
LEAP-Seq n confirming:6478
LEAP-Seq n nonconfirming:209
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:CACACTGCCGAATACCACAC
Suggested primer 2:CTCCCGGGTAGTGCTTATCA