| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.157760 |
| Chromosome: | chromosome 10 |
| Location: | 1593124 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429400 | OPR45,MCG1 | petG mRNA stabilization factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGATGCAACGGGCGCTAGAGGCAGCTGG |
| Internal bar code: | ATACACGTCCCGGGAAAGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 557 |
| LEAP-Seq percent confirming: | 50.9758 |
| LEAP-Seq n confirming: | 862 |
| LEAP-Seq n nonconfirming: | 829 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAACGCGTTGGGTTAGAG |
| Suggested primer 2: | CTAGCACCATCACCGTCGT |