Insertion junction: LMJ.RY0402.157782_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.157782
Chromosome:chromosome 16
Location:4507658
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g667350 CAV6 (1 of 2) PF00520//PF08016//PF16905 - Ion transport protein (Ion_trans) // Polycystin cation channel (PKD_channel) // Voltage-dependent L-type calcium channel, IQ-associated (GPHH); Voltage-gated Ca2+ channel, alpha subunit CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGTGCTGCTGTGGGCCGAGTACGACGAC
Internal bar code:CTGAGGTAGGGCTTCTTTTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:162
LEAP-Seq percent confirming:99.4609
LEAP-Seq n confirming:738
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AGTCACTGGTCATGTCGCTG
Suggested primer 2:COULD_NOT_FIND_PRIMER