Insertion junction: LMJ.RY0402.157783_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.157783
Chromosome:chromosome 9
Location:2069740
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g394050 CPLD19 Zinc finger family protein; (1 of 1) PTHR22883:SF91 - PROTEIN S-ACYLTRANSFERASE 12-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTGCTCAGGATTGGAGAAAGCTACCCCT
Internal bar code:GCGGCCCAGCGTCGCGGCCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:152
LEAP-Seq percent confirming:99.8634
LEAP-Seq n confirming:3656
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCTGTGTCTCCAACGCTC
Suggested primer 2:ATACGTCCTCCCAAACCTCC