| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.157799 |
| Chromosome: | chromosome 8 |
| Location: | 2514210 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g372000 | BAF3,CGLD6,CGLD11 | chloroplast ATPase CF1 assembly factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATGCGCCATCGGCAGCTTTCCCGCGCGT |
| Internal bar code: | GGATGCGATCATCTTCCGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 867 |
| LEAP-Seq percent confirming: | 98.0485 |
| LEAP-Seq n confirming: | 1658 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTCTAGTGAAGCACGAGG |
| Suggested primer 2: | CTGCCTCCATACACATGCAC |