| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.157803 |
| Chromosome: | chromosome 12 |
| Location: | 8530504 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547700 | TTL14 | (1 of 14) PF03133 - Tubulin-tyrosine ligase family (TTL); Putative tubulin tyrosine ligase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTAGGATCCAGGGTGCGCCTCTCAGGGA |
| Internal bar code: | CGCGCCTACTCAGTCTCCACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 460 |
| LEAP-Seq percent confirming: | 89.87 |
| LEAP-Seq n confirming: | 15073 |
| LEAP-Seq n nonconfirming: | 1699 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATTGATGATGGTGGTTCC |
| Suggested primer 2: | GTCCATTGGCAGTTTCGTTT |