Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.157822 |
Chromosome: | chromosome_17 |
Location: | 6206019 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g741950 | BBS1 | Bardet-Biedl syndrome-1 associated protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATTGACCCTATGTGATGTATGATAGGGCTT |
Internal bar code: | CGTCGCGTTTTTATGCGCCGGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 681 |
LEAP-Seq percent confirming: | 99.8099 |
LEAP-Seq n confirming: | 1575 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGTCTCATGACACCATCACC |
Suggested primer 2: | CGAGCATTTCTATGTGGGGT |