| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.157824 |
| Chromosome: | chromosome 9 |
| Location: | 1926007 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395200 | (1 of 1) PF04087 - Domain of unknown function (DUF389) (DUF389) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGATCATCCCCCCGCTCCTGAGGCCTGT |
| Internal bar code: | GGGCGACTGAACTGGCAAAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1060 |
| LEAP-Seq percent confirming: | 99.4397 |
| LEAP-Seq n confirming: | 6921 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTACAGCAAGGTCACCGA |
| Suggested primer 2: | GTTACATGGGGATCATTCGG |