| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157828 |
| Chromosome: | chromosome 9 |
| Location: | 2967624 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387750 | (1 of 100) IPR001810 - F-box domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCCCATCGCCGTACTGGCCTGGGCTTT |
| Internal bar code: | GACGGGGGCACGCGGGCGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 289 |
| LEAP-Seq percent confirming: | 95.4128 |
| LEAP-Seq n confirming: | 416 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCCTGGGCTTAGTTGTA |
| Suggested primer 2: | GAGATGCAGCAGCAAGACAG |