Insertion junction: LMJ.RY0402.157832_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.157832
Chromosome:chromosome_11
Location:2702183
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre11.g476850 DRC4,PF2 Component of dynein regulatory complex antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):CATAGCCTGAACCCGAAGCCATGGGGTGGA
Internal bar code:ACGTGGGTCCTAATCTTCGAAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:78
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TACCCTCCTACACCCAGTGC
Suggested primer 2:CTCGACACACATATCCACGG