| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.157872 |
| Chromosome: | chromosome 12 |
| Location: | 4834266 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524800 | (1 of 4) IPR005819 - Histone H5 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGCGCGAGCGCGAGCAGTTCGGTCAAC |
| Internal bar code: | CGGCAGTATGGGTGGGTACAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 324 |
| LEAP-Seq percent confirming: | 99.8081 |
| LEAP-Seq n confirming: | 1040 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTGTCTGTCTAAGCACGG |
| Suggested primer 2: | GTACAGAGGGAACAACCCGA |