| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.157888 |
| Chromosome: | chromosome 1 |
| Location: | 5046983 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034900 | CDPK5 | (1 of 4) PTHR24347:SF138 - PROTEIN F32D8.1; Calcium/calmodulin-dependent protein kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCCTCAGCCCGCAGAATGGCCAACTGC |
| Internal bar code: | TGGTGCTGGTATGGCGGGTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 824 |
| LEAP-Seq percent confirming: | 95.5224 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATATGGGCTCGTGGGTATG |
| Suggested primer 2: | AATCAATCGTCAAAGCGTCC |