| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157906 |
| Chromosome: | chromosome 1 |
| Location: | 3371955 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021400 | SPL13 | Pre-mRNA splicing factor; (1 of 1) PTHR16196//PTHR16196:SF0 - CELL CYCLE CONTROL PROTEIN CWF25 // PRE-MRNA-SPLICING FACTOR CWC25 HOMOLOG | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCTTAGCGGGCGCTTGCAGGCAAATTGT |
| Internal bar code: | GCTGACCTGCGGTCACTGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 748 |
| LEAP-Seq percent confirming: | 99.714 |
| LEAP-Seq n confirming: | 1046 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGAGGTTTCAAATACGGT |
| Suggested primer 2: | TTGCTATGTGCTATGCAGCC |