| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157915 |
| Chromosome: | chromosome 17 |
| Location: | 2671681 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g717750 | TRL10,TRX24 | (1 of 1) PTHR21148//PTHR21148:SF25 - PHOSDUCIN-RELATED // PHOSDUCIN-LIKE PROTEIN 1; Thioredoxin-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGTGTCTGTGGCCCAAACTCATTCGGC |
| Internal bar code: | TCGTGGAATCGACCTTTCGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 526 |
| LEAP-Seq percent confirming: | 99.8527 |
| LEAP-Seq n confirming: | 1356 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGATGTGAAGATGGTGATG |
| Suggested primer 2: | TCCATTTCTTTCATCCCGAG |