| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.157922 |
| Chromosome: | chromosome 13 |
| Location: | 3010689 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g584350 | (1 of 100) IPR001810 - F-box domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTACTGCCGGCCGCGCCTCCGCAGATGGC |
| Internal bar code: | TCCTCGCTTGTTGGTTTGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 940 |
| LEAP-Seq percent confirming: | 99.7972 |
| LEAP-Seq n confirming: | 2461 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTCAGGGGAGATATCCGA |
| Suggested primer 2: | CCCTGCTGGTATACGGAAGA |