| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157944 |
| Chromosome: | chromosome 12 |
| Location: | 4588752 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522550 | FLA8,KIN2-1 | Kinesin II Motor Protein; (1 of 2) K10394 - kinesin family member 3/17 (KIF3_17) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCCTCGCATCAGCAGCGCTAACGCTGGA |
| Internal bar code: | CTGTGGCGACCAAGTGATCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 289 |
| LEAP-Seq percent confirming: | 99.7647 |
| LEAP-Seq n confirming: | 424 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACAAGGACTCGGGTGTAT |
| Suggested primer 2: | CCTCATTGATCTTGGGCTGT |