Insertion junction: LMJ.RY0402.157965_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.157965
Chromosome:chromosome 7
Location:3658373
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g337300 DSK2,DYRK2,DYRKP1,DYRKP,STD1 Dual-Specificity Tyrosine-Regulated Protein Kinase involved in starch degradation; (1 of 2) PTHR24058//PTHR24058:SF23 - DUAL SPECIFICITY PROTEIN KINASE // SERINE/THREONINE KINASE-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTCGCGTGAGATCTCTTTGGGCCCTTCC
Internal bar code:GGTCGCTTACTGCCCGACAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:724
LEAP-Seq percent confirming:98.448
LEAP-Seq n confirming:3235
LEAP-Seq n nonconfirming:51
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:ACCGCGAGGTATTGTACTGG
Suggested primer 2:GGCAAGGCTTGACAACAACT