| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157981 |
| Chromosome: | chromosome 7 |
| Location: | 101652 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g312850 | (1 of 1) PTHR31621:SF1 - DUF679 DOMAIN MEMBRANE PROTEIN 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAGCCCAACATCTGGCGTGGAGGGCCCA |
| Internal bar code: | ACGTCGTCGATGGACTGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 846 |
| LEAP-Seq percent confirming: | 99.7086 |
| LEAP-Seq n confirming: | 1711 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCTCTCCCTACCCGAGAC |
| Suggested primer 2: | AAAATGTCAACTTCGGACGG |