| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.157982 |
| Chromosome: | chromosome 5 |
| Location: | 2707716 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGGCCCATCTACAAGCAGCACAACCTG |
| Internal bar code: | GTTATTTTGAACGAGTTCTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 980 |
| LEAP-Seq percent confirming: | 99.4483 |
| LEAP-Seq n confirming: | 721 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAATATTTGGCTGCCGAG |
| Suggested primer 2: | CTTGTCCGTACATCGTGTGG |