| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.157991 |
| Chromosome: | chromosome 12 |
| Location: | 7174387 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558700 | FAP67 | Flagellar Associated Protein 67; (1 of 4) K00940 - nucleoside-diphosphate kinase (ndk, NME) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGCAAGTCCGCAACGCCACACCCTATTT |
| Internal bar code: | ACTAGCCCCTGGAGTTGTCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 133 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCTGGAGGTGTACAAGGG |
| Suggested primer 2: | GTGCACCTCTCATGCAGTGT |