| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.158007 |
| Chromosome: | chromosome 6 |
| Location: | 7136601 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g297400 | (1 of 1) 1.3.1.74 - 2-alkenal reductase (NAD(P)(+)) / NADPH:2-alkenal alpha,beta-hydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGCCGGACACGACCAGACACACCTGTG |
| Internal bar code: | GGTATGTGAGGAACTTAACACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 772 |
| LEAP-Seq percent confirming: | 99.6173 |
| LEAP-Seq n confirming: | 2603 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGCGTCTGTACATTTTCT |
| Suggested primer 2: | GGTAAATACCAGCCCAAGCA |