| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.158033 |
| Chromosome: | chromosome 7 |
| Location: | 3609651 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g336750 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTACTGCCGCGCATCGGCGGCCCCGCAGC |
| Internal bar code: | TCCATCTGAAGTTCGGACACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 318 |
| LEAP-Seq percent confirming: | 99.7358 |
| LEAP-Seq n confirming: | 5662 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCCTAGCTCTGTAGCCCC |
| Suggested primer 2: | GTGAGCGAGAGGGTTTGTTT |