| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158035 |
| Chromosome: | chromosome 9 |
| Location: | 5050996 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g398326 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAATCTGGCATCCTGCTTCTGAGCTCGGG |
| Internal bar code: | TACGACGAATTATACAGGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 801 |
| LEAP-Seq percent confirming: | 99.6465 |
| LEAP-Seq n confirming: | 3664 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGAGGATTCTGACTGCGA |
| Suggested primer 2: | ATGATGGGGACATAGCGAAG |