| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158036 |
| Chromosome: | chromosome 2 |
| Location: | 236466 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g074800 | CYG9 | (1 of 1) IPR000700//IPR001054//IPR029787 - PAS-associated, C-terminal // Adenylyl cyclase class-3/4/guanylyl cyclase // Nucleotide cyclase; Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTCCACTGCTTAATACCAAGAACAGCA |
| Internal bar code: | TCGCTGCGCCTTATCAGCACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 925 |
| LEAP-Seq percent confirming: | 99.762 |
| LEAP-Seq n confirming: | 2096 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTCGCTAGCACACACAG |
| Suggested primer 2: | ATGCACCCAGACTACGAACC |