| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.158065 |
| Chromosome: | chromosome 16 |
| Location: | 549799 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692550 | MSH4 | DNA mismatch repair protein, MutS homolog; (1 of 1) K08740 - DNA mismatch repair protein MSH4 (MSH4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGGCAGATAGAGGCAGTGCAATCACAA |
| Internal bar code: | GCTTCCCGGGTCAAGCCCGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 409 |
| LEAP-Seq percent confirming: | 25.6515 |
| LEAP-Seq n confirming: | 1565 |
| LEAP-Seq n nonconfirming: | 4536 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGAAGGAGATGGAGGGAC |
| Suggested primer 2: | ACTCATCATCAACGCCATCA |