| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.158089 |
| Chromosome: | chromosome 2 |
| Location: | 519921 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077061 | (1 of 1) PTHR22911//PTHR22911:SF27 - ACYL-MALONYL CONDENSING ENZYME-RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCGGCGCGTGGGTGTGGGGCCTGCAGCA |
| Internal bar code: | GCGGCGACGGCCGCTGTGTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 759 |
| LEAP-Seq percent confirming: | 85.443 |
| LEAP-Seq n confirming: | 270 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCTAGCTCATGCTGATG |
| Suggested primer 2: | TACAACACGAATACGGGCAA |