| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.158097 |
| Chromosome: | chromosome 8 |
| Location: | 4858564 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g384700 | DMA3 | (1 of 1) PF06325 - Ribosomal protein L11 methyltransferase (PrmA) (PrmA); Putative DNA methylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAGCCGCCACCTGGCGGGACAGGGCGCG |
| Internal bar code: | GATCTCGCGCATGGGATGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 510 |
| LEAP-Seq percent confirming: | 98.9071 |
| LEAP-Seq n confirming: | 181 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGAGAGGCACACGTTCA |
| Suggested primer 2: | GCGTGTGGAGCTCTTGTGTA |