| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.158124 |
| Chromosome: | chromosome 3 |
| Location: | 508477 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145227 | (1 of 1) K12199 - vacuolar protein sorting-associated protein VTA1 (VTA1, LIP5) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATAAGGAATAGTCGTAATAGTTTGAGAC |
| Internal bar code: | CTCCTTGAGTTTCCTACCTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 367 |
| LEAP-Seq percent confirming: | 99.4712 |
| LEAP-Seq n confirming: | 3950 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCCAGCTGGTTGAGTGTG |
| Suggested primer 2: | CCTCAGGTTCGCTCAAAGTC |