| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.158125 |
| Chromosome: | chromosome 1 |
| Location: | 1795387 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g009676 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTATGATGTGGGTTTCAGTGGATGCAATG |
| Internal bar code: | AGACGAAGGGTGCTCCACATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 889 |
| LEAP-Seq percent confirming: | 73.6396 |
| LEAP-Seq n confirming: | 2693 |
| LEAP-Seq n nonconfirming: | 964 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCTCACGCAGGTGATGTG |
| Suggested primer 2: | ATCGCAGGAACGTACAAACC |