| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.158140 |
| Chromosome: | chromosome 2 |
| Location: | 5129706 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105150 | (1 of 1) 4.1.1.19 - Arginine decarboxylase / L-arginine carboxy-lyase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGAAGTGGGCGCACACGAGTGCTGAGGC |
| Internal bar code: | CCGATGTACTCTTTACCTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1132 |
| LEAP-Seq percent confirming: | 99.5199 |
| LEAP-Seq n confirming: | 1451 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAGCGACGTATGTGAGT |
| Suggested primer 2: | GGGCTAATGTCTGAAGCGAG |