| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158153 |
| Chromosome: | chromosome 3 |
| Location: | 5453677 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185300 | GAK1,GALK1 | (1 of 1) 2.7.1.46 - L-arabinokinase; Galactokinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTTCCAACCACCGTCTCCCTACCTACCT |
| Internal bar code: | AGGCGAGGCTGCGTTGGTGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 540 |
| LEAP-Seq percent confirming: | 99.7215 |
| LEAP-Seq n confirming: | 358 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGGGTGTGGAGGTGTCT |
| Suggested primer 2: | CAGTACGACCGTGACATTGG |