| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158156 |
| Chromosome: | chromosome 8 |
| Location: | 487448 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358800 | (1 of 2) 5.1.1.17 - Isopenicillin-N epimerase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCACGGCAATCATGTCCTGAACCGACA |
| Internal bar code: | GTTTATGGTATAGCGCGGCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 936 |
| LEAP-Seq percent confirming: | 99.2431 |
| LEAP-Seq n confirming: | 1180 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCACACCGCAAAACACAA |
| Suggested primer 2: | CTACCCTTCTGAGGCACAGC |