| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158172 |
| Chromosome: | chromosome 2 |
| Location: | 3950394 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096551 | (1 of 230) IPR017986 - WD40-repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAATGAAACTTCCCCACACCCGCCCACCT |
| Internal bar code: | GTATTGGATCTCACGGAAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 702 |
| LEAP-Seq percent confirming: | 47.0282 |
| LEAP-Seq n confirming: | 5333 |
| LEAP-Seq n nonconfirming: | 6007 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGAGGGTGAAGACGAAG |
| Suggested primer 2: | ACAATCTTGGGGAACAGCAC |