Insertion junction: LMJ.RY0402.158177_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.158177
Chromosome:chromosome 7
Location:2248624
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g327450 DNJ34 DnaJ-like protein; (1 of 1) PTHR24078//PTHR24078:SF201 - DNAJ HOMOLOG SUBFAMILY C MEMBER // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCCGCTGTTGCTGCTTGCTGAAGGCGGA
Internal bar code:GTGCTAGCGCTCGGATGTCGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:323
LEAP-Seq percent confirming:93.1232
LEAP-Seq n confirming:650
LEAP-Seq n nonconfirming:48
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AATTGTGTCGCAATCAACCA
Suggested primer 2:CAACGCTGGATACAAGCAGA